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Mumbai, Maharashtra, India
Last Updated: 2 Mar, 2015

Rapid Malaria Stain Kit



Rapid Malaria Stain Kit

We are offering to our medical clients this high quality Rapid Malaria Stain Kit (JSB I & II). It is used in hospitals, clinics and nursing homes for several purposes. Customers need to use this kit cautiously. Its procedure states that the two coplin jars or wide mouth bottles need to be filled with rapid-malaria stain -I and II. As its major principle, polychromatic methylene blue and eosin stains specifically to basophilic and acidophilic cellular elements to demonstrate blood cells and hemoparasites.

 

Composition information:

 

  • Stains supplied: Cat. no. HE 807
  • Preparation of working reagent: All reagents are ready to use

 

Rapid-malaria JSBI 500 ml:

 

  • Methylene blue: 4.3 m Mol/L
  • Sulphuric acid: 1%
  • Potassium dichromate: 0.5 gms
  • Phosphate buffer: 0.1 m Mol
  • Contains polychromating materials, preservatives and stabilizers

 

Rapid-malaria JSB II 500 ml:

 

  • Eosin: 4.3 m Mol/L
  • Phosphate: 0.1 m Mol/L
  • Contains preservatives and stabilizers
  • Easyfix 50 ml
  • Peripheral smear fixative

 

Stability:

 

  • All reagents are stable between 25 to 30 degree C for 60 months
  • Rapid-malaria fixative must be stored tight capped away from heat and fire
  • Rapid-malaria JSB stain are exhausted on prolonged use over 150 smears
  • Advised to discard rapid-malaria JSB Stain as and when the smear gets lighter stain

 

Samples:

 

  • Peripheral blood samples on clean glass slides may be collected upon febrile episodes from susceptible patients
  • Avoid anticoagulated samples

 

Thin Smear:

 

  • A drop of blood can be spread with a fine edge spreader
  • Dry the smear at air and fix it with fixative or rectified spirit within 4 hours
  • Fixed smear can be stored for longer period without much variation in staining result
  • Preferable to stain all smears as early as possible

 

Thick Smears:

 

  • In mass or conclusive screening it is preferable to prepare thick smears by collecting 1-2 drop of blood on a clean glass slide and spread it to the shape of a coin
  • Dry at air, add few drops of distilled water to the slide to cover the smears and wait for 1-2 minutes to dehemoglobinized the smear
  • Carefully remove water and dry at air
  • Fix the smear

 



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